In Marvel's Spider-Man, the player takes on the role of an experienced Peter Parker, who has been Spider-Man for eight years. The game's narrative follows Peter as he faces a new threat to New York City, orchestrated by the villain Mister Negative. As Spider-Man, players must navigate the city, fight crime, and balance their superhero duties with their personal life. The game features a mix of combat, stealth, and puzzle-solving, with key moments involving other characters like Mary Jane Watson and Miles Morales. The story is set in a unique universe known as Earth-1048, inspired by the long-running comic book mythology and various adaptations in other media.